Aktivitas Antioksidan Fraksi Larut Etil Asetat dari Ekstrak Akar Tabar Kedayan (Aristolochia papilifolia Ding Hou)

Novermawati; Iswahyudi; Islamudin Ahmad; Muhammad Amir Masruhim

doi:10.70392/xh39pf05

Authors

Novermawati Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia Author
Iswahyudi Departemen Penelitian dan Pengembangan, PT. Borneo Riseta Naturafarm, Kutai Kartanegara, Kalimantan Timur, Indonesia Author
Islamudin Ahmad Laboratorium Riset dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia Author
Muhammad Amir Masruhim Program Studi Magister Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia Author

DOI:

https://doi.org/10.70392/xh39pf05

Keywords:

Tabar Kedayan root (Aristolochia papillfolia Ding hou), antioxidant, fraction, inhibition concentration of 50% (IC50)

Abstract

Kedayan root is a medicinal plant that is traditionally used by the community as medicine, especially as an antidote to poison. This research aimed to determine the antioxidant activity of the ethyl acetate soluble fraction of Tabar Kedayan root extract (Aristolochia papillfolia Ding hou). The fractionation method used is solid-liquid extraction using ethyl acetate solvent to obtain a soluble fraction of ethyl acetate, then vacuum liquid chromatography fractionation using the eluent ratio n-hexane: ethyl acetate and obtain fraction A, fraction B, fraction C, fraction D, fraction E, fraction F. Testing of antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method spectrophotometrically. The data obtained was analyzed using linear regression to determine IC₅₀. The results of the C fraction test on the ethyl acetate soluble fraction of Tabar Kedayan roots (A. papillfolia) have antioxidant activity with an IC₅₀ value of 139.11 ppm.